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f4 80  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec f4 80
    F4 80, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f4/F4%2F80+Antibody%2C+anti-mouse%2C+REAfinity/pmc13694607-279-15-19
    Average 96 stars, based on 208 article reviews
    f4 80 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Airborne 1 O 2 Delivery via a Superhydrophobic Dressing as a Pathway to Next-Generation Wound Therapies, an in Vivo Murine Burn Model Study.
    Article Snippet: .. Cells were stained for 30 min at 4◦C in he dark with fluorochrome-conjugated antibodies as follows: nti-F4/80-PE-Vio770 (mouse, clone REA126, Miltenyi Biotec, :50) to identify total macrophages, anti-CD206-PerCP/Cy5.5 rat monoclonal, clone C068C2, BioLegend, 1:50) to identify nti-inflammatory M2 macrophages, and anti-TNF-α-FITC (rat onoclonal, clone MP6-XT22, BioLegend, 1:100) to detect pronflammatory cytokine-producing cells. ..

    Article Title: Murine peritoneal macrophages undergo female-specific remodeling with aging
    Article Snippet: .. For primary MACS-purified peritoneal macrophages, we also included an additional staining step with F4/80-APC (Miltenyi Biotec #130-116-547) for downstream data filtering. ..

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation
    Article Snippet: .. Following Fc receptor blocking (anti-CD16/CD32, 10 min, 4 °C), spleen and PBMC cells were stained for 30 min at 4 °C in the dark with the following antibodies: F4/80-FITC (REAfinityTM, Miltenyi Biotec), Ly6G-PE (clone 1A8, BD PharmingenTM), and CD11b-APC (clone M1/70, BioLegend). .. After staining, cells were washed twice and fixed with IC Fixation Buffer (eBioscience, Cat. 00-8222-49, Invitrogen) for 15 min at 4 °C in the dark.

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation.
    Article Snippet: .. Following Fc receptor blocking (anti-CD16/CD32, 10 min, 4 °C), spleen and PBMC cells were stained for 30min at 4 °C in the dark with the following antibodies: F4/80-FITC (REAfinityTM, Miltenyi Biotec), Ly6G-PE (clone 1A8, BD PharmingenTM), and CD11b-APC (clone M1/70, BioLegend). .. After staining, cells were washed twice and fixed with IC Fixation Buffer (eBioscience, Cat. 00-8222-49, Invitrogen) for 15 min at 4 °C in the dark.

    Article Title: Murine peritoneal macrophages undergo female-specific remodeling with aging
    Article Snippet: .. After FcR-blocking, macrophages were stained using F4/80-APC (1:50; Miltenyi Biotec #130-116-547) and CD11b-Vioblue (1:50; Miltenyi Biotec #130-113-238) antibodies according to the manufacturer’s instructions. .. Stained cells were analyzed by flow cytometry on the Miltenyi Biotec MACSQuant Analyzer 10.

    other:

    Article Title: Bacterial microcompartments and energy metabolism drive gut colonization by Bilophila wadsworthia.
    Article Snippet: Each librarywas sequencedwith ~8.7million 150bppairedend reads at the Genewiz sequencing centre (UK).

    Article Title: Bacterial microcompartments and energy metabolism drive gut colonization by Bilophila wadsworthia
    Article Snippet: A < 30 mg piece from the caecum of six animals per group was dissected and incubated in RNAlater (Thermo Fisher Scientific) at 4 °C overnight to stabilise the RNA.

    Magnetic Cell Separation:

    Article Title: Murine peritoneal macrophages undergo female-specific remodeling with aging
    Article Snippet: .. For primary MACS-purified peritoneal macrophages, we also included an additional staining step with F4/80-APC (Miltenyi Biotec #130-116-547) for downstream data filtering. ..

    Blocking Assay:

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation
    Article Snippet: .. Following Fc receptor blocking (anti-CD16/CD32, 10 min, 4 °C), spleen and PBMC cells were stained for 30 min at 4 °C in the dark with the following antibodies: F4/80-FITC (REAfinityTM, Miltenyi Biotec), Ly6G-PE (clone 1A8, BD PharmingenTM), and CD11b-APC (clone M1/70, BioLegend). .. After staining, cells were washed twice and fixed with IC Fixation Buffer (eBioscience, Cat. 00-8222-49, Invitrogen) for 15 min at 4 °C in the dark.

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation.
    Article Snippet: .. Following Fc receptor blocking (anti-CD16/CD32, 10 min, 4 °C), spleen and PBMC cells were stained for 30min at 4 °C in the dark with the following antibodies: F4/80-FITC (REAfinityTM, Miltenyi Biotec), Ly6G-PE (clone 1A8, BD PharmingenTM), and CD11b-APC (clone M1/70, BioLegend). .. After staining, cells were washed twice and fixed with IC Fixation Buffer (eBioscience, Cat. 00-8222-49, Invitrogen) for 15 min at 4 °C in the dark.



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